The determination of isoflavones and coumestrol by capillary electrophoresis

J. Moravcová and T. Kleinová
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J. Moravcová: Institute of Chemical Technology - Department of Chemistry of Natural Compounds, Prague, Czech Republic
T. Kleinová: Institute of Chemical Technology - Department of Chemistry of Natural Compounds, Prague, Czech Republic

Czech Journal of Food Sciences, 2001, vol. 19, issue 4, 132-138

Abstract: The separation of six isoflavones (biochanin A, isoformononetin, formononetin, prunetin, daidzein and genistein) and coumestrol on an uncoated fused-silica capillary electrophoresis column was optimised using alkaline borate buffer as electrolyte and DAD detection. A baseline separation of all analytes except a pair, formononetin-biochanin A was achieved at pH 10.5 in 25 min. Detection limits were low (0.1 µg/ml) and the linearity of the detector response was established in the concentration range 0.4-60 µg/ml (180 µg/ml for coumestrol). Coumestrol was synthesized and the carbon signals in 13C-NMR spectrum of both coumestrol and di-O-acetylcoumestrol were assigned for the first time using two-dimensional HMQC technique.

Keywords: phytoestrogens; food; feed; separation; isoflavones; coumestrol; electrophoresis (search for similar items in EconPapers)
Date: 2001
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:19:y:2001:i:4:id:6596-cjfs

DOI: 10.17221/6596-CJFS

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