Unusual behavior of natural polyphosphates during IMAC

M. Kmínková, A. Prošková and J. Kučera
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M. Kmínková: Food Research Institute Prague, Prague, Czech Republic
A. Prošková: Food Research Institute Prague, Prague, Czech Republic
J. Kučera: Food Research Institute Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2001, vol. 19, issue 5, 161-165

Abstract: The atypical course of IDA-ferric (Imino-Di-Acetyl-agarose saturated with ferric ions) of two polyphosphorylated compounds - phytic acid and Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster obtained by the way of pancreatic hydrolysis of bovine casein was detected. The spectral data (for phytic acid) and iron content (for polyphosphate cluster) indicate the strong bond of ferric ions to these polyphosphorylated compounds. The data shows these compounds to bind ferric ion stronger even in comparison with IDA (Imino-Di-Acetate) itself. Therefore attention is to be paid to all IMAC (Immobilized-Metal-Ion-Affinity-Chromatography) of natural compounds containing strong chelating groups or several chelating groups in vicinity.

Keywords: phytic acid; Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster; affinity chromatography; immobilized metal ion; IMAC; IDA-ferric; atypical adsorption (search for similar items in EconPapers)
Date: 2001
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:19:y:2001:i:5:id:6601-cjfs

DOI: 10.17221/6601-CJFS

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