Beta-galactosidase activity in psychrotrophic microorganisms and their potential use in food industry

P. Karasová, V. Spiwok, Š. Malá, B. Králová and N.J. Russell
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P. Karasová: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,
V. Spiwok: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,
Š. Malá: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,
B. Králová: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,
N.J. Russell: Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague,

Czech Journal of Food Sciences, 2002, vol. 20, issue 2, 43-47

Abstract: Twenty-one psychrotrophic resp. psychrophilic bacterial strains were screened for presence of b-galactosidase activity which showed 8 of them. b-Galactosidase activity of these strains was determined for 2 substrates - synthetic substrate (ONPG) and lactose - and also temperature profile of this enzyme was measured. b-Galactosidase from Arthrobacter sp. C2-2 not only proved the typical properties of cold-active enzyme, but it also preferred lactose as a substrate. Therefore, it was chosen for further isolation and purification and was found that it contains two b-galactosidase isoenzymes. One of them had strong preference for lactose and was able to catalyse transglycosylation reactions at low temperature. It has, thus, potential use in food technology.

Keywords: psychrotrophic microorganism; cold-active enzyme; b-galactosidase; lactose hydrolysis; transglycosylation (search for similar items in EconPapers)
Date: 2002
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DOI: 10.17221/3508-CJFS

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