Molecular cloning and characterisation of alpha subunit of H+-ATPase in Lactobacillus casei Zhang

Xia Chen, Mei Yang, Zhihong Sun, Wenjun Liu, Tiansong Sun, He Meng and Heping Zhang
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Xia Chen: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, P.R.China
Mei Yang: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, P.R.China
Zhihong Sun: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, P.R.China
Wenjun Liu: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, P.R.China
Tiansong Sun: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, P.R.China
He Meng: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R.China
Heping Zhang: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, P.R.China

Czech Journal of Food Sciences, 2009, vol. 27, issue 1, 49-54

Abstract: The acid tolerance is an important property of Lactic acid bacteria as potential probiotics. H+-ATPase is considered a key gene in several bacteria with the ability of acid tolerance. We cloned and sequenced the full length cDNA of alpha subunit of H+-ATPase gene in Lactobacillus casei Zhang, which had been isolated from traditional home-made koumiss in Inner Mongolia of China. The results showed that the respective cDNA sequence is composed of 1530 nucleotides and codes a putative protein including 509 amino acids. In addition, we also reconstructed the phylogenic trees for H+-ATPase gene based on amino acids sequences of diverse strains of Lactic acid bacteria.

Keywords: H+-ATPase; clone; sequence; Lactobacillus casei Zhang; phylogenic trees (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:1:id:134-2008-cjfs

DOI: 10.17221/134/2008-CJFS

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