Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

Muhammad Arfan, Hazrat Amin, Magdalena Karamać, Agnieszka Kosińska, Wiesław Wiczkowski and Ryszard Amarowicz
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Muhammad Arfan: Instutute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
Hazrat Amin: Instutute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
Magdalena Karamać: Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
Agnieszka Kosińska: Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
Wiesław Wiczkowski: Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
Ryszard Amarowicz: Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland

Czech Journal of Food Sciences, 2009, vol. 27, issue 2, 109-117

Abstract: Phenolic compounds were extracted from Mallotus philippinensi bark using methanol. Six fractions (I-VI) were separated from the extract on a Sephadex LH-20 column using ethanol and acetone-water as the mobile phases and were evaluated for their total antioxidant activity, antiradical activity against DPPH* (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents in the fractions were determined. The content of total phenolics in the fractions ranged from 54 mg/g (fraction I) to 927 mg/g (fraction VI). Condensed tannins were detected in fractions II-VI. Total antioxidant activity (TAA) of phenolic fractions of Mallotus philipinensis bark extract ranged from 0.58 mmol Trolox/g (fraction I) to 6.82 mmol Trolox/g (fraction IV). Fraction IV also showed the strongest antiradical activity against DPPH* and reducing power. Several phenolic constituents in the fractions were detected by RP-HPLC using a gradient solvent system with UV-DAD detection.

Keywords: Mallotus philippinensis; bark; extract; natural antioxidants; phenolic compounds; tannins; antioxidant activity; antiradical activity (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:2:id:1056-cjfs

DOI: 10.17221/1056-CJFS

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