Influence of glycation and pepsin hydrolysis on immunoreactivity of albumin/globulin fraction of herbicide resistant wheat line

András Nagy, Katarzyna Marciniak-Darmochwał, Stanisław Krawczuk, Dagmara Mierzejewska, Henryk Kostyra and Éva Gelencsér
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András Nagy: Department of Biology, Central Food Research Institute, Budapest, Hungary
Katarzyna Marciniak-Darmochwał: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
Stanisław Krawczuk: Faculty of Biology, University of Warmia and Mazury, Olsztyn, Poland
Dagmara Mierzejewska: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
Henryk Kostyra: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland
Éva Gelencsér: Department of Biology, Central Food Research Institute, Budapest, Hungary

Czech Journal of Food Sciences, 2009, vol. 27, issue 5, 320-329

Abstract: The aim of this study was to investigate the influence of non-enzymatic glycosylation on the immunogenic properties of soluble wheat proteins. Albumin/globulin fractions of herbicide resistant wheat line were non-enzymatically glycosylated using glucose for seven days at 37°C. The changes in their structures and immunoreactivity were then determined. The protein fractions were also hydrolysed with pepsin to determine the resistance to digestion. Albumin/globulin fractions before and after non-enzymatic glycosylation were analysed using o-phthaldialdehyde method and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The immunoreactivity of the protein fractions was determined using enzyme-linked immunosorbent assay methods, as well as affinity chromatography. The soluble wheat proteins showed smaller amounts of available α-amino groups after non-enzymatic glycosylation, and were stronger immunogens after glycation, but their antigenicity was not been affected significantly. However, pepsin hydrolysis of wheat proteins decreased their immunoreactivity.

Keywords: non-enzymatic glycosylation; glycation; pepsin hydrolysis; immunoreactivity (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:5:id:48-2008-cjfs

DOI: 10.17221/48/2008-CJFS

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