Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation

M. Dušková, A. Španová, V. Dráb and B. Rittich
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M. Dušková: Department of Milk Hygiene and Technology, University of Veterinary and Pharmaceutical Sciences Brno, 612 42 Brno, Czech Republic
A. Španová: Institute of Experimental Biology, Masaryk University, 602 00 Brno, Czech Republic
V. Dráb: Dairy Research Institute Ltd., 160 00 Praha, Czech Republic

Czech Journal of Food Sciences, 2009, vol. 27, issue SpecialIssue1, S366-S368

Abstract: In this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as a DNA probe for the screening of homologous DNA sequences in 7 Lactococcus lactis subsp. lactis strains from the Culture Collection of Dairy Microorganisms (CCDM). Dot blot hybridisation and hybridisation of colonies were used for DNA/DNA hybridisation. It was shown that 6 tested strains of Lactococcus lactis subsp. lactis have genes encoding nisin. One strain had probably a defective gene encoding nisin.

Keywords: Lactococcus lactis subsp .lactis; screening; nisin; gene; PCR probe; DNA/DNA hybridisation (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue1:id:603-cjfs

DOI: 10.17221/603-CJFS

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