Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii

Igor Hochel and Jiří Škvor
Additional contact information
Igor Hochel: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
Jiří Škvor: Department of Medical Biophysics, 1 st Medical Faculty, Charles University in Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2009, vol. 27, issue SpecialIssue2, SII66-SII74

Abstract: An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739T were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 × 105 cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.

Keywords: Enterobacter sakazakii; heat-labile antigen; IgG; ELISA; cross-reactivity (search for similar items in EconPapers)
Date: 2009
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjfs.agriculturejournals.cz/doi/10.17221/206/2009-CJFS.html (text/html)
http://cjfs.agriculturejournals.cz/doi/10.17221/206/2009-CJFS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue2:id:206-2009-cjfs

DOI: 10.17221/206/2009-CJFS

Access Statistics for this article

Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová, Ph.D.

More articles in Czech Journal of Food Sciences from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().