The expression of selected genes encoding enterotoxins in Staphylococcus aureus strains

Lukáš Valihrach, Kateřina Demnerová, Renata Karpíšková and Ivana Melenová
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Lukáš Valihrach: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
Kateřina Demnerová: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
Renata Karpíšková: National Institute of Public Health Prague, Brno, Czech Republic
Ivana Melenová: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2009, vol. 27, issue SpecialIssue2, SII56-SII65

Abstract: Staphylococcus aureus is an important food-borne pathogen, which produces many toxic substances that cause a variety of illnesses. Some strains of S. aureus produce thermostable enterotoxins that can be responsible for alimentary intoxication. The aim of this work was to establish a protocol for the study of 9 enterotoxin genes expression (sea-sej). First, a method for the detection of genes encoding enterotoxins was established and then a method for the determination of the expression of these genes was optimised, using a range of the PCR techniques (multiplex, touchdown and real-time). The expression of staphylococcal enterotoxin genes was evaluated both qualitatively and quantitatively. In present study were used S. aureus strains from culture collections as well as those newly-isolated from raw milk samples. The obtained results indicate the various expression of the different genes for enterotoxin. However the main benefit of this work is the established protocol for the study of enterotoxin gene expression, which can provide a better understanding of the conditions for the enterotoxins production.

Keywords: staphylococcal enterotoxin; gene expression; multiplex PCR; real-time PCR; mRNA analysis (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue2:id:208-2009-cjfs

DOI: 10.17221/208/2009-CJFS

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