PCR-based identification of toxinogenic Fusarium species

Iva Lacmanová, Jarmila Pazlarová, Marta Kostelanská and Jana Hajšlová
Additional contact information
Iva Lacmanová: Department of Biochemistry and Microbiology and
Jarmila Pazlarová: Department of Biochemistry and Microbiology and
Marta Kostelanská: Department of Food Analysis, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
Jana Hajšlová: Department of Food Analysis, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2009, vol. 27, issue SpecialIssue2, SII90-SII94

Abstract: Aim of this study was to develop sensitive PCR assay for mycotoxin producing Fusarium species. Strains Fusarium oxysporum 4199 and Fusarium culmorum 4044 were used as representatives of this group. Primers JB chosen to demonstrate the affiliation to genus Fusarium were derived from ITS region of rDNA. Gene from trichothecene pathway Tri4 was employed to design primers for toxin biosynthesis. Specificity of PCR based on JB primers was tested on DNA isolated from F. culmorum 4044, F. oxysporum 4199, Aspergillus oryzae 4002 and Mucor circinelloides 4018, Trichoderma sp. Both Fusarium species gave positive reaction, while the later ones did not react. Primers based on Tri4 highly specific sequences were giving positive reaction only with DNA from F. culmorum 4044 and F. oxysporum 4199. DNA isolated from six samples of contaminated wheat grains gave positive result on the presence of genus Fusarium and mycotoxines by optimised PCR protocol using JB and Tri4 primers. The results corresponded to LC/MS analysis that was established quantitatively in all samples to ascertain the amount and type of fusarious mycotoxines.

Keywords: toxinogenic Fusarium; trichothecenes; PCR; JB primers; Tri4 primers (search for similar items in EconPapers)
Date: 2009
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjfs.agriculturejournals.cz/doi/10.17221/634-CJFS.html (text/html)
http://cjfs.agriculturejournals.cz/doi/10.17221/634-CJFS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue2:id:634-cjfs

DOI: 10.17221/634-CJFS

Access Statistics for this article

Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová, Ph.D.

More articles in Czech Journal of Food Sciences from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().