Interferences of PCR effectivity: importance for quantitative analyse

Jan Hodek, Jaroslava Ovesná and Ladislav Kučera
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Jan Hodek: Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic
Jaroslava Ovesná: Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic
Ladislav Kučera: Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic

Czech Journal of Food Sciences, 2009, vol. 27, issue SpecialIssue2, SII42-SII49

Abstract: Importance of the Polymerase chain reaction (PCR) have already crossed the border of mere target DNA sequence present or absence analysis. For number analyses e.g. Genetically Modified Organisms (GMOs) or gene expression assesment the DNA quantification is demanded. Real-time (or quantitative) PCR is the most used tool for nucleic acids quantification. PCR efficiency has relevant importance on DNA quantification - it should be almost same for each PCR and its value should varied between 90-100%. There are a lot of PCR enhancers and inhibitors well known. We described impact of used DNA solvent and used laboratory plastic on real-time PCR efficiency.

Keywords: real-time PCR; DNA quantification; PCR efficiency; GMO analysis (search for similar items in EconPapers)
Date: 2009
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:27:y:2009:i:specialissue2:id:677-cjfs

DOI: 10.17221/677-CJFS

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