Kinetics of hydrolysis of egg white protein by pepsin

Chang-Qing Ruan, Yu-Jie Chi and Rui-Dong Zhang
Additional contact information
Chang-Qing Ruan: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China
Yu-Jie Chi: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China
Rui-Dong Zhang: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, P. R. China

Czech Journal of Food Sciences, 2010, vol. 28, issue 5, 355-363

Abstract: Taking into account the enzyme inactivation and substrate inhibition, the bioreaction mechanism and kinetics characteristic of egg white protein (EWP) enzymatic hydrolysis by pepsin were investigated. A logarithmic equation h = (1/b) ln (1 + abt) indicating the relationship between the degree of hydrolysis (DH) and time was established. For EWP-pepsin system, the reaction mechanism could be deduced from a series of experimental results at different temperatures, pH values, substrate concentrations, and enzyme concentrations. The reaction kinetics and thermodynamic constants (KS = 3916.5 g/l, k2 = 17 202.86 min-1, kd = 21 962.03, Ea = 56.89 kJ/mol, Ed = 51.99 kJ/mol) were responsible for the empirical equations. The results of nonlinear regression of the proposed kinetic model agreed with the experimental data, i.e. the average relative error was less than 5%. As a conclusion, the kinetic equations can be used to fit the enzymatic hydrolysis process of egg white protein and to optimise the operating parameters of bioactive peptides preparation for the bioreactor design.

Keywords: egg white protein; pepsin; enzymatic hydrolysis; kinetics; bioactive peptides (search for similar items in EconPapers)
Date: 2010
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjfs.agriculturejournals.cz/doi/10.17221/228/2009-CJFS.html (text/html)
http://cjfs.agriculturejournals.cz/doi/10.17221/228/2009-CJFS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:28:y:2010:i:5:id:228-2009-cjfs

DOI: 10.17221/228/2009-CJFS

Access Statistics for this article

Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová Ph.D.

More articles in Czech Journal of Food Sciences from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().