Utilization of DNA microarrays for detection and identification of selected Fusarium species from the Czech Republic

Lucie Pavlátová, David Novotný, Jan Hodek, Jana Chrpová and Jaroslava Ovesná
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Lucie Pavlátová: Department of Molecular Biology,
David Novotný: Department of Mycology and
Jan Hodek: Department of Molecular Biology,
Jaroslava Ovesná: Department of Molecular Biology,

Czech Journal of Food Sciences, 2011, vol. 29, issue SpecialIssue, S93-S101

Abstract: Fusarium is a serious phytopathogenic fungal genus with producting of many kinds of highly toxic secondary metabolites - mycotoxins. The consumption of Fusarium contaminated food and feed can cause dangerous mycotoxicoses both in humans and animals, therefore the detection of a wider range of Fusarium species in the samples of crops is very important. The aim of our work was to test the reliability of detection and identification of three Fusarium species in infected wheat grains by DNA microarrays versus classical mycological methods and by specific PCR. The in-house DNA microarrays for the detection and identification of the selected Fusarium species by using oligonucleotides probes were prepared. For hybridisation on DNA microarrays fluorescent labelled PCR products were used of part of the translation elongation factor 1 alpha. The conditions of hybridisation were optimised on fungal template DNA. The method of DNA microarrays was verified on artificially infected samples of wheat and tested on unknown infected wheat samples with simultaneous analysis by classical mycological methods and by specific PCR.

Keywords: DNA microarrays; fluorescent labelling; Fusarium; oligonucleotide probe; PCR (search for similar items in EconPapers)
Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:29:y:2011:i:specialissue:id:307-2011-cjfs

DOI: 10.17221/307/2011-CJFS

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Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová, Ph.D.

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