Genetic determinants of mycotoxin synthesis in genus Fusarium

Hana Havránková, Jarmila Pazlarová and Jaroslava Ovesná
Additional contact information
Hana Havránková: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
Jarmila Pazlarová: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
Jaroslava Ovesná: Department of Molecular Biology, Crop Research Institute, Prague-Ruzyně, Czech Republic

Czech Journal of Food Sciences, 2011, vol. 29, issue SpecialIssue, S86-S93

Abstract: The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins. In our study, we focused on the analysis by Q-PCR of two genes expression - Tri4, Tri10 - involved in the production of Fusarium mycotoxin deoxynivalenol. Reference genes β-tub and UBC were found to be suitable for the expression level normalisation using the program geNorm. The level of expression of the target genes was analysed on four isolates of Fusarium graminearum grown in vitro. In all four isolates, Tri10 gene expression level was lower than that of Tri4 gene expression.

Keywords: Fusarium; trichothecene biosynthesis; Tri4; Tri10; real-time PCR; geNorm (search for similar items in EconPapers)
Date: 2011
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjfs.agriculturejournals.cz/doi/10.17221/314/2011-CJFS.html (text/html)
http://cjfs.agriculturejournals.cz/doi/10.17221/314/2011-CJFS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:29:y:2011:i:specialissue:id:314-2011-cjfs

DOI: 10.17221/314/2011-CJFS

Access Statistics for this article

Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová, Ph.D.

More articles in Czech Journal of Food Sciences from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().