Simultaneous determination of the residues of fourteen quinolones and fluoroquinolones in fish samples using liquid chromatography with photometric and fluorescence detection

Florentina Cañada-Cañada, Anunciacion Espinosa-Mansilla, Ana Jiménez Girón and Arsenio Muñoz de la Peña
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Florentina Cañada-Cañada: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain
Anunciacion Espinosa-Mansilla: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain
Ana Jiménez Girón: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain
Arsenio Muñoz de la Peña: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain

Czech Journal of Food Sciences, 2012, vol. 30, issue 1, 74-82

Abstract: A chromatographic method is described for assaying fourteen quinolones and fluoroquinolones (pipemidic acid, marbofloxacin, norfloxacin, ciprofloxacin, danofloxacin, lomefloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, nalidixic acid, flumequine, and pyromidic acid) in fish samples. The samples were extracted with m-phosphoric acid/acetonitrile mixture (75:25, v/v), purified, and preconcentrated on ENV + Isolute cartridges. The determination was achieved by liquid chromatography using C18 analytical column. A mobile phase composed of mixtures of methanol-acetonitrile-10mM citrate buffer at pH 4.5, delivered under optimum gradient program, at a flow rate of 1.5 ml/min, allows accomplishing the chromatographic separation in 26 minutes. For the detection were used serial UV-visible diode-array at 280 nm and 254 nm and fluorescence detection at excitation wavelength/emission wavelength: 280/450, 280/495, and 280/405 nm. The detection and quantification limits were between 0.2-9.5and 0.7-32 µg/kg, respectively. The procedure was applied to the analysis of spiked salmon samples at two different concentration levels (50 µg/k and 100 µg/kg). Mean recoveries of fluoroquinolones from the salmon samples ranged from 50% to 102%, depending on the analyte.

Keywords: chromatographic method; UV detectecton; salmon (search for similar items in EconPapers)
Date: 2012
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:30:y:2012:i:1:id:12-2010-cjfs

DOI: 10.17221/12/2010-CJFS

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