Characterisation of antilisterial bacteriocin-like substance produced by Enterococcus mundtii

Kateřina Solichová, Ivana Složilová, Iva Jebavá, Barbora Uhrová and Milada Plocková
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Kateřina Solichová: Department of Dairy and Fat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
Ivana Složilová: Department of Dairy and Fat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
Iva Jebavá: Department of Dairy and Fat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
Barbora Uhrová: Department of Dairy and Fat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic
Milada Plocková: Department of Dairy and Fat Technology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2012, vol. 30, issue 1, 89-97

Abstract: The antilisterial activity of E. mundtii 1282 strain and its cell-free neutralised supernatant was observed against five persistent L. monocytogenes strains using the agar spot method. The enterococcal metabolite was consequently characterised as a proteinaceous substance - the bacteriocin-like substance, which was heat-stable at heating to 100°C for 30 min, stable at pH 2-12, and still active after eight-week long storage at 6°C and -20°C. The bacteriocin-like substance reached the highest activity of 6400 AU/ml after ten hours of cultivation of E. mundtii 1282 strain, when it was at the stationary phase of its growth. E. mundtii 1282 strain produced the bacteriocin-like substance: in BHI broth with pH 4-6, at the cultivation temperature 12-45°C, and in BHI broth with 1-6% (w/w) NaCl.

Keywords: Enterococcus; enterocin-like substance; Listeria monocytogenes; persistent (search for similar items in EconPapers)
Date: 2012
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:30:y:2012:i:1:id:278-2010-cjfs

DOI: 10.17221/278/2010-CJFS

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