Bacterial biofilms resist oxidising agents due to the presence of organic matter

Zoran Jaglic, Dana Červinková, Hana Vlková, Elleni Michu, Gabriela Kunová and Vladimír Babák
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Zoran Jaglic: Veterinary Research Institute, Brno, Czech Republic,
Dana Červinková: Veterinary Research Institute, Brno, Czech Republic,
Hana Vlková: Veterinary Research Institute, Brno, Czech Republic,
Elleni Michu: Veterinary Research Institute, Brno, Czech Republic,
Gabriela Kunová: Dairy Research Institute, Prague, Czech Republic
Vladimír Babák: Veterinary Research Institute, Brno, Czech Republic,

Czech Journal of Food Sciences, 2012, vol. 30, issue 2, 178-187

Abstract: This study evaluated the susceptibility of planktonic and biofilm cells of Staphylococcus spp. (n = 87), Klebsiella spp. (n = 30), and Escherichia coli (n = 74) isolates originating from food contact surfaces of milk and meat processing plants to benzalkonium chloride (BAC), sodium hypochlorite (NaClO), chloramine B (CAB), and peracetic acid (PAA). Bacterial growth and reduction of viable cells in the presence of disinfectants were determined in tryptone soya broth (TSB) and water, respectively. Biofilm positive isolates (n = 73) were tested for the presence of selected qac genes. Unlike BAC, chlorine‑based disinfectants and PAA were poorly efficient in TSB, especially in the case of biofilms. However, when tested in water, the efficacy of NaClO, CAB and PAA substantially increased, which was particularly evident in biofilms. In water, staphylococcal biofilms were even more susceptible to CAB than planktonic cells. A part (23.3%) of the biofilm positive staphylococci carried the qac genes but did not express an increased resistance to BAC. This study showed that bacterial biofilms protected with organic matter could be one of the main reasons for disinfection failure.

Keywords: food safety; MIC; dairy; sanitation; biocide; hygiene (search for similar items in EconPapers)
Date: 2012
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:30:y:2012:i:2:id:214-2011-cjfs

DOI: 10.17221/214/2011-CJFS

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