Characterisation of polyphenol oxidase from Melissa officinalis L. subsp. officinalis (lemon balm)

Serap Doğan, Yasemin Ayyildiz, Mehmet Doğan, Ümran Alan and Mehmet Emin Di̇ken
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Serap Doğan: Department of Biology
Yasemin Ayyildiz: Department of Biology
Mehmet Doğan: Department of Chemistry, Faculty of Science and Literature, University of Balikesir, Çağiş-Balikesir, Turkey
Ümran Alan: Department of Biology
Mehmet Emin Di̇ken: Department of Biology

Czech Journal of Food Sciences, 2013, vol. 31, issue 2, 156-165

Abstract: Polyphenol oxidase (PPO) from Melissa officinalis L. subsp. officinalis (lemon balm) was partially purified by ammonium sulphate precipitation and dialysis; and then it was characterised in detail in terms of pH and temperature optima, thermal stability, kinetic parameters, and inhibition properties. Based on experimental results, it was found out that (i) the optimum pH and temperature values of PPO were 6.5, 4.0, and 8.5 and 40, 50, and 60°C for catechol, 4-methylcatechol and pyrogallol substrates, respectively; (ii) the best substrate was pyrogallol due to the highest Vmax/Km value, followed by catechol and 4-methylcatechol; (iii) enzyme activity decreased due to heat denaturation of the enzyme with increasing temperature and inactivation time for all substrates; (vi) gallic acid and l-glutamic acid did not inhibit PPO; and (v) the most effective inhibitor was glutathione. Furthermore, the phenolic and protein contents of lemon balm extract were also determined according to the Folin-Ciocalteu and Bradford methods, respectively.

Keywords: lemon balm; protein; enzyme kinetics; inhibition (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:31:y:2013:i:2:id:288-2011-cjfs

DOI: 10.17221/288/2011-CJFS

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