Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

Orlando G. Nagel, Maria Pilar Molina and Rafael L. Althaus
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Orlando G. Nagel: Department of Basic Science, Faculty of Veterinary Sciences, National University of Litoral, Esperanza, Argentina
Maria Pilar Molina: Department of Animal Science, Polytechnic University of Valencia, Valencia, Spain
Rafael L. Althaus: Department of Basic Science, Faculty of Veterinary Sciences, National University of Litoral, Esperanza, Argentina

Czech Journal of Food Sciences, 2013, vol. 31, issue 6, 627-632

Abstract: We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (24-1). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

Keywords: B. subtilis; microbial inhibition method; bioassay; experimental design; logistic regression model (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:31:y:2013:i:6:id:494-2011-cjfs

DOI: 10.17221/494/2011-CJFS

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