Influence of salts on selective coagulation of whey proteins and their application in the isolation of β-lactoglobulin

Jana Hanušová, Miroslava Mihulová, Lenka Diblíková and Ladislav Čurda
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Jana Hanušová: Department of Dairy, Fat and Cosmetics, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
Miroslava Mihulová: Department of Dairy, Fat and Cosmetics, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
Lenka Diblíková: Department of Dairy, Fat and Cosmetics, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic
Ladislav Čurda: Department of Dairy, Fat and Cosmetics, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2014, vol. 32, issue 1, 77-81

Abstract: Whey proteins are an important constituent of milk, especially whey from cheese manufacture and have many valuable functional properties such as foaming and emulsifying ability or gel formation. Some whey proteins are sensitive to salt content in a solution. High or low salt content may lead to selective coagulation of these proteins. A part of whey proteins was precipitated by addition of 7% (wt) NaCl and β-lactoglobulin and caseinomacropeptide remained in the supernatant. It was necessary to demineralise the supernatant by electrodialysis for the selective coagulation of caseinomacropeptide from this material. Subsequently, ethanol was added and pH was adjusted. This reduction of the ionic strength and the addition of ethanol induced the selective precipitation of caseinomacropeptide (91.4% from the original amount of CMP). β-lactoglobulin of 91% purity remained in the solution.

Keywords: whey protein concentrate; electrodialysis; precipitation of caseinomacropeptide; separation of proteins; salting out (search for similar items in EconPapers)
Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:32:y:2014:i:1:id:524-2012-cjfs

DOI: 10.17221/524/2012-CJFS

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