Quantification of bee-derived peptide defensin-1 in honey by competitive enzyme-linked immunosorbent assay, a new approach in honey quality control

Ivana Valachová, Marcela Bučeková and Juraj Majtán
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Ivana Valachová: Department of Zoology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic
Marcela Bučeková: Institute of Zoology and 3Laboratory of Molecular Apidology and Apitherapy, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic
Juraj Majtán: Institute of Zoology and 3Laboratory of Molecular Apidology and Apitherapy, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic

Czech Journal of Food Sciences, 2016, vol. 34, issue 3, 233-243

Abstract: We established and evaluated a polyclonal antibody based competitive enzyme-linked immunosorbent assay for the quantification of defensin-1 in honey. The assay showed an inhibitory concentration (IC50) value of 111.5 ± 15.41 ng/ml with a detection limit of 7.8125 ng/ml. The regaining of defensin-1 in spiked 'artificial honey' was between 87.05 and 112.96% with relative standard deviation less than 9.2%. Sensitivity and specificity of the test were experimentally validated on a sample of 20 different honeys. The antibacterial activity of these honey samples showed a significant concentration-dependent correlation with the production of defensin-1 (n = 20; r = -0.6598; P = 0.0016). The assay provides a specific and sensitive method for the screening of defensin-1 in honey. The method to detect honeybee-derived proteins in honey is a promising approach to verifying the authenticity of honey. The defensin-1 ELISA could also be used for the rapid screening of honeys suitable for medicinal purposes.

Keywords: competitive ELISA; honey adulteration; antibacterial peptides; Apis mellifera (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:34:y:2016:i:3:id:422-2015-cjfs

DOI: 10.17221/422/2015-CJFS

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