Effects of a bilberry preparation on selected cell lines of the digestive system

Kamila Borowiec, Dominik Szwajgier, Anna Olejnik, Katarzyna Kowalska and Zdzisław Targoński
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Kamila Borowiec: Department of Biotechnology, Human Nutrition and Science of Food Commodities, Faculty of Food Science and Biotechnology, University of Life Sciences, Lublin, Poland
Dominik Szwajgier: Department of Biotechnology, Human Nutrition and Science of Food Commodities, Faculty of Food Science and Biotechnology, University of Life Sciences, Lublin, Poland
Anna Olejnik: Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition Poznań University of Life Sciences, Poznań, Poland
Katarzyna Kowalska: Department of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition Poznań University of Life Sciences, Poznań, Poland
Zdzisław Targoński: Department of Biotechnology, Human Nutrition and Science of Food Commodities, Faculty of Food Science and Biotechnology, University of Life Sciences, Lublin, Poland

Czech Journal of Food Sciences, 2016, vol. 34, issue 4, 300-305

Abstract: Bilberry is a valuable wild forest fruit harvested in many countries in Europe. The biological activities of bilberry include antioxidant, anticancer, antiviral, antibacterial, and anticholinesterase activities. This study examines the protective effects of a bilberry (BB) preparation on IEC-6, Caco-2, and HepG2 cell lines. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to study the cytotoxicity of BB. The genotoxicity was determined using single-cell microgel electrophoresis. The Ames test was employed to assay bilberry mutagenicity. No significant effects of BB (12.5-100 µg dry mass/ml) were observed on the viability of IEC-6, Caco-2, and HepG2 cells. There were no differences in the percentage of DNA in the comet tail between the cells treated with BB (100 µg dry mass/ml) and the control cells. However, a significant reduction of oxidative DNA damage in the HepG2 cells was found. BB exhibited neither mutagenic nor promutagenic effects. Our results suggest that bilberry can be a potential tool in the prevention of chronic diseases, without any undesired effects on the cells of the gastrointestinal tract.

Keywords: Vaccinium myrtillus L.; cytotoxicity; functional food; genotoxicity; mutagenicity (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:34:y:2016:i:4:id:375-2015-cjfs

DOI: 10.17221/375/2015-CJFS

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