Enzyme-linked immunosorbent assay for simultaneous detection of two fungicides kresoxim-methyl and trifloxystrobin in oranges

Wei Yang, Jie Zhu, Ming-Qi Liu and Xian-Jun Dai
Additional contact information
Wei Yang: College of Life Sciences, China Jiliang University, Hangzhou, P.R. China
Jie Zhu: College of Life Sciences, China Jiliang University, Hangzhou, P.R. China
Ming-Qi Liu: College of Life Sciences, China Jiliang University, Hangzhou, P.R. China
Xian-Jun Dai: College of Life Sciences, China Jiliang University, Hangzhou, P.R. China

Czech Journal of Food Sciences, 2016, vol. 34, issue 5, 429-438

Abstract: To assemble an indirect competitive enzyme-linked immunosorbent assay (ELISA) for estimating two strobilurin fungicides at the same time, the hapten was synthesised which contained the common active group, (E)-2-(2-bromo-phenyl)-2-(methoxyimino) acetic acid methyl ester (OEBr) in kresoxim-methyl and trifloxystrobin. The immunogen and coating antigen were respectively prepared through conjugating the above-mentioned hapten with BSA and OVA by the mixed anhydride and activated ester methods, and polyclonal antibodies were produced by immunised rabbits. An enzyme-linked immunosorbent assay was developed for simultaneous detection of kresoxim-methyl and trifloxystrobin. In ELISA, the antiserum showed high affinity and sensitivity to kresoxim-methyl and trifloxystrobin, and their IC50 value and detection limit (expressed as IC10) were 14.7 and 0.0044 µg/ml, respectively, for kresoxim-methyl, and 22.9 and 0.014 µg/ml, respectively for trifloxystrobin. The cross-reaction rate was below 0.1% for other strobilurin fungicides. Recovery study of ELISA from spiked samples of homogenised peeled oranges (final concentrations of 100, 10, and 1 µg/ml) resulted in recovery levels in the range of 82-104%.

Keywords: kresoxim-methyl; trifloxystrobin; polyclonal antibody; ELISA; recovery rate (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjfs.agriculturejournals.cz/doi/10.17221/275/2015-CJFS.html (text/html)
http://cjfs.agriculturejournals.cz/doi/10.17221/275/2015-CJFS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:34:y:2016:i:5:id:275-2015-cjfs

DOI: 10.17221/275/2015-CJFS

Access Statistics for this article

Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová, Ph.D.

More articles in Czech Journal of Food Sciences from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().