Immobilisation of endoinulinase on polyhydroxybutyrate microfibers

Milos Beran, Jitka Pinkrová, Marian Urban and Josef Drahorád
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Milos Beran: Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic
Jitka Pinkrová: Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic
Marian Urban: Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic
Josef Drahorád: Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic

Czech Journal of Food Sciences, 2016, vol. 34, issue 6, 541-546

Abstract: Due to the health benefits associated with the consumption of prebiotic short-chain fructooligosaccharides (sc-FOS) and inulooligosaccharides (IOS), there is increased interest in the use of these compounds in food products. We have developed a new biocatalyst for the production of FOS and IOS by inulin hydrolysis. Endoinulinase from Aspergillus niger Inulinase® Novozym 960 (Novozymes) was immobilised on polyhydroxybutyrate (PHB) nanofibres and microfibres by hydrophobic interactions. The PHB fibres were prepared by centrifugal spinning. FOS and IOS profiles were determined by ion-exchange chromatography with the Rezex RSO-Oligosaccharide column. The biocatalyst had very good activity and stability after repeated applications. It can be used in biocatalytic membrane reactors for the production of prebiotic oligosaccharides.

Keywords: inulin; fructooligosaccharide; inulooligosaccharide; inulinase; nanofibres; PHB (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:34:y:2016:i:6:id:72-2016-cjfs

DOI: 10.17221/72/2016-CJFS

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