Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7

Junhua Wang, Shuangzhi Zhao, Jiying Qiu, Qingxin Zhou, Xiaoyong Liu, Xue Xin, Danyang Guo, Tatyana G. Yudina, Yifen Wang$, Hua Sun, Xiangyan Chen and Leilei Chen
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Junhua Wang: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Shuangzhi Zhao: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Jiying Qiu: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Qingxin Zhou: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Xiaoyong Liu: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Xue Xin: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Danyang Guo: Department of Microbiology, Moscow State University, Moscow, Russia
Tatyana G. Yudina: Department of Microbiology, Moscow State University, Moscow, Russia
Yifen Wang$: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Hua Sun: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Xiangyan Chen: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China
Leilei Chen: Institute of Agro-food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, P.R. China

Czech Journal of Food Sciences, 2017, vol. 35, issue 2, 113-121

Abstract: Bacillus amyloliquefaciens NCPSJ7 could secrete extracellular antimicrobial substances, showing potent antifungal activities. An active peptide AFP3 was isolated from the fermentation supernatant. After chromatography, the purified peptide was tested for the fungicidal activity, molecular mass, and stability. The results indicated that the peptide with a molecular mass of around 3.3 kDa, showed discernible inhibition of the pathogen Fusarium oxysporum f. sp. niveum with the minimum fungicidal concentration of 31 µg/ml. It also exhibited excellent inhibition of some representative pathogenic fungi at a low concentration. Moreover, the peptide remained active at a wide range of temperatures and pH. Ion Na+ may even increase the antifungal activities. At the same time, the peptide could well tolerate the treatment with trypsin. Electron microscopy was used to investigate the effect of the peptide on the pathogens. The peptide inhibited the growth of pathogens by disrupting the integrity of the hyphal membranes, resulting in their lysis. The potent fungicidal activities and stability made the peptide be a candidate for a biopreservative.

Keywords: antifungal peptide; mechanism; stability; biopreservative (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:35:y:2017:i:2:id:163-2016-cjfs

DOI: 10.17221/163/2016-CJFS

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