Honey Sold Directly by Producers in the Silesian Region of Poland as a Source of Clostridium botulinum Types A, B, E, and F

Beata Wysok, Joanna Wojtacka, Robert Karczmarczyk, Agnieszka Wiszniewska-Łaszczych, Małgorzata Gomółka-Pawlicka, Joanna Szteyn and Katarzyna Liedtke
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Beata Wysok: Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, in Olsztyn, Poland
Joanna Wojtacka: Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, in Olsztyn, Poland
Robert Karczmarczyk: Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
Agnieszka Wiszniewska-Łaszczych: Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, in Olsztyn, Poland
Małgorzata Gomółka-Pawlicka: Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, in Olsztyn, Poland
Joanna Szteyn: Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, in Olsztyn, Poland
Katarzyna Liedtke: Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, in Olsztyn, Poland

Czech Journal of Food Sciences, 2017, vol. 35, issue 3, 194-199

Abstract: The level of contamination of honey with Clostridium botulinum spores is considered as an indicator of the adequacy of hygienic practices during collection, extraction, and subsequent processing. A total of 39 honey samples purchased directly from beekeepers at outdoor markets and from small amateur apiaries in Silesia were analysed for Clostridium botulinum spores. The samples were prepared using a dilution centrifugation method and cultured in parallel in cooked meat medium (CMM) and tripticase peptone glucose yeast (TPGY) enrichment broths. Identification of C. botulinum toxin types A, B, E, and F was performed with the use of a multiplex PCR method. The analysis showed six (15.4%) samples to be contaminated with C. botulinum spores. The major serotypes detected were type A - in two (5.1%) and type B - in two (5.1%) honey samples, respectively. Types E and F were found in 1 (2.6%) and 1 (2.6%) positive honey sample analysed, respectively.

Keywords: anaerobic bacteria; bee product; PCR; spores (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:35:y:2017:i:3:id:376-2016-cjfs

DOI: 10.17221/376/2016-CJFS

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