Validation of a UHPLC-ESI-MS/MS method for anthocyanidin quantification in potato tubers
Miloslav Šulc,
Marie Eliášová,
Zora Kotíková and
Jaromír Lachman
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Marie Eliášová: Department of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Zora Kotíková: Department of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Jaromír Lachman: Department of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
Czech Journal of Food Sciences, 2017, vol. 35, issue 3, 223-228
Abstract:
The development of bioanalytical methods has become challenging due to sample complexity, requirements for method reliability, and speed of analysis with triple quadrupole LC-MS/MS used widely for the routine analysis of biological materials. The article presents the method development and validation results for pelargonidin and malvidin in potato tubers. The developed method uses a short C18 column, is able to measure all six common anthocyanidins, uses a binary mobile phase with acetonitrile and water both with added 1% formic acid, ESI ionisation in positive mode, 3-h hydrolysis with 2.7 M methanolic HCl at 90°C. For pelargonidin and malvidin, the method shows high recovery of 98-100%, intra-day repeatability of 6.7-17.9% (depending on the analyte and concentration level), uncertainty below 20%, and uses quadratic calibration.
Keywords: acid hydrolysis; method development; Solanum tuberosum L (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:35:y:2017:i:3:id:389-2016-cjfs
DOI: 10.17221/389/2016-CJFS
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