Determining the optimal method for DNA isolation from fruit jams

Tereza Sovová, Barbora Křížová and Jaroslava Ovesná
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Barbora Křížová: Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic
Jaroslava Ovesná: Department of Molecular Genetics, Crop Research Institute, Prague, Czech Republic

Czech Journal of Food Sciences, 2018, vol. 36, issue 2, 126-132

Abstract: DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods - two kits: DNeasy® Plant Mini Kit and NucleoSpin® Food, and the CTAB method - for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration and optical density were analysed using UV spectrophotometry and the amplifiability of the obtained DNA was evaluated using a PCR assay targeting a sequence coding for chloroplast tRNA-Leu. Samples isolated using the NucleoSpin® Food kit contained non-amplifiable DNA in eight cases, and samples isolated using the CTAB method could not be quantified. The DNeasy® Plant Mini Kit thus proved to be the most suitable method, since well-amplifiable DNA was obtained for all the analysed samples.

Keywords: DNA amplifiability; DNA extraction; food analysis; food quality (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:36:y:2018:i:2:id:340-2017-cjfs

DOI: 10.17221/340/2017-CJFS

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