Investigation of hepatitis A and E viruses in mussels collected from the Bosphorus, in Istanbul, Turkey - short communication

Huseyin Yilmaz, Utku Cizmecigil, Eda Altan Tarakci, Ozge Aydin, Aysun Yilmaz, Mehmet Calicioglu, Gurhan Ciftcioglu, Ali Aydin, Kamil Bostan, Tansel Sireli, Mine Guzel, Asiye Karakullukcu, Bekir Kocazeybek, Wim van der POEL, Juergen Richt and Nuri Turan
Additional contact information
Huseyin Yilmaz: Department of Virology
Utku Cizmecigil: Department of Virology
Eda Altan Tarakci: Department of Virology
Ozge Aydin: Department of Virology
Aysun Yilmaz: Department of Virology
Mehmet Calicioglu: Department of Food Hygiene and Technology, Veterinary Faculty, University of Firat, Elazig, Turkey
Gurhan Ciftcioglu: Department of Food Hygiene and Technology, Veterinary Faculty, University of Istanbul, Istanbul, Turkey
Ali Aydin: Department of Food Hygiene and Technology, Veterinary Faculty, University of Istanbul, Istanbul, Turkey
Kamil Bostan: Department of Food Hygiene and Technology, University of Aydin, Istanbul, Turkey
Tansel Sireli: Department of Food Hygiene and Technology, Veterinary Faculty, University of Ankara, Ankara, Turkey 6Biruni Laboratories, Centro lab., Istanbul, Turkey
Asiye Karakullukcu: Department of Microbiology, Cerrahpasa Faculty of Medicine, University of Istanbul, Istanbul, Turkey
Bekir Kocazeybek: Department of Microbiology, Cerrahpasa Faculty of Medicine, University of Istanbul, Istanbul, Turkey
Wim van der POEL: Central Veterinary Institute, Lelystad, The Netherlands
Juergen Richt: Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, USA
Nuri Turan: Department of Virology

Czech Journal of Food Sciences, 2018, vol. 36, issue 3, 215-220

Abstract: Hepatitis A and E viruses (HAV and HEV) are recognised as food- and water-borne infections and shellfish are a well-documented source of these viruses. The presence of HAV and HEV in mussels has not previously been investigated in Bosphorus, Istanbul, Turkey, and therefore the aim of this study was to investigate the frequency of HAV and HEV in mussels collected from this location. A total of 92 pooled samples representing 736 mussels originating along the Bosphorus coast were collected from fish distributors. RNA was extracted using the RNeasy Plant Mini Kit and a TaqMan real-time RT-PCR was performed using primers specific to HAV and HEV. Amongst the 92 pooled samples tested, 3 (3.3%) were found to be positive for HAV by the TaqMan real-time RT-PCR. No HEV was detected in any of the mussels tested. A 174 bp product was observed on gel electrophoresis with HAV positive samples detected by SYBR Green real-time PCR after reverse transcription. This product was sequenced and confirmed to contain HAV sequences by alignment using the data in GenBank. This is the first report describing the presence of HAV in mussels harvested in the Bosphorus, Istanbul, Turkey, indicating the potential contamination of aquatic environment and risk to public health.

Keywords: hepatitis; mussels; Turkey; virus; real-time RT-PCR (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:36:y:2018:i:3:id:233-2017-cjfs

DOI: 10.17221/233/2017-CJFS

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