Simultaneous quantifications of four purine derivatives biomarkers in cow milk by SPE HPLC-DAD

Mihaela Vlassa, Filip Miuța and Cătălin Dragomir
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Mihaela Vlassa: Analytical Chemistry Department, Raluca Ripan Institute for Research in Chemistry, Babeş-Bolyai University, Cluj-Napoca, Romania
Filip Miuța: Analytical Chemistry Department, Raluca Ripan Institute for Research in Chemistry, Babeş-Bolyai University, Cluj-Napoca, Romania
Cătălin Dragomir: National Institute for Research and Development for Biology and Animal Nutrition, Balotesti, Romania

Czech Journal of Food Sciences, 2021, vol. 39, issue 2, 122-130

Abstract: In this study, simultaneous quantification of allantoin, uric acid, xanthine, and hypoxanthine in cow milk by solid phase extraction (SPE) and high performance liquid chromatography-diode array detection (HPLC-DAD) method was perform. Five different SPE cartridges were tested in order to evaluate the isolation of purine derivatives (PD) from cow milk. Chromatography was carried out on ODS-2 Hypersil column and 0.05 M (NH4)2HPO4 buffer solution (pH = 7.76) as mobile phase. The HPLC-DAD validated method showed a linearity with regression coefficients higher than 0.999 and the limits of detection and quantification with values in the range 0.09-0.74 µg mL-1 and 0.27-2.24 µg mL-1, respectively. The method showed good precision with a relative standard deviation (RSD) below 4.48%, while the accuracy ranged from 95.34 to 104.47% for all analytes. The best recovery degree of PD by SPE were obtained on Strata SCX cartridge for xanthine (87.79%) and hypoxanthine (89.02%); on Strata NH2 for allantoin (35.09%) and on Strata C8 for uric acid (101.08%). Finally, the HPLC-DAD method with SPE on SCX cartridges was applied to quantify the PD in a batch of thirty cow milk samples.

Keywords: solid phase extraction; high performance liquid chromatography-diode array detection; ruminant milk; PD; method validation (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:39:y:2021:i:2:id:297-2020-cjfs

DOI: 10.17221/297/2020-CJFS

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Czech Journal of Food Sciences is currently edited by Ing. Zdeňka Náglová, Ph.D.

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