Extraction optimisation and lipid-lowering activity of Auricularia heimuer polysaccharides

Xiang Hui Kong, Yin Peng Ma, Yu Pan, Wei Jiang, Ding Jin Li, Xi Jun Chen, Guo Li Yang, Zhen Hua Duan and Jie Chi Zhang
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Xiang Hui Kong: Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
Yin Peng Ma: Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
Yu Pan: Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
Wei Jiang: Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China
Ding Jin Li: Institute of Food Research, Hezhou University, Hezhou, China
Xi Jun Chen: Heilongjiang Johnsun Biological Engineering Co., Ltd., Harbin, China
Guo Li Yang: Heilongjiang Johnsun Biological Engineering Co., Ltd., Harbin, China
Zhen Hua Duan: Institute of Food Research, Hezhou University, Hezhou, China
Jie Chi Zhang: Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, China

Czech Journal of Food Sciences, 2021, vol. 39, issue 6, 452-459

Abstract: Assessments of molecular weight distribution and activity/efficacy of Auricularia heimuer polysaccharides (AAP) are of substantial significance for its extraction process optimisation. In the present study, single-factor orthogonal test and response surface methodology were employed to optimise extraction conditions of AAP. Furthermore, a rat hyperlipidaemia model was established to compare the lipid-lowering activity of polysaccharides obtained by three extraction methods. Conditions for enzymatic hydrolysis were optimised as pH 5.0, 1% cellulase, 2.5% substrate concentration and enzymolysis time of 1.5 h, leading to an up to 31.8% polysaccharide yield and 89.13% of polysaccharides within the molecular weight range of 5 000 Da to 10 000 Da. The results of animal experiments showed that the lipid-lowering activity of enzymolysis-extracted polysaccharides was significantly higher than that of water- and ultrasonic-extracted ones (P < 0.01). So the present study revealed that enzymatic hydrolysis-extracted polysaccharides showed the strongest hypolipidaemia activity, providing a basis for the development of A. heimuer-based functional foods and drugs.

Keywords: hot water extraction; ultrasonic-assisted; cellulase-assisted; molecular weight; HPLC (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:39:y:2021:i:6:id:146-2020-cjfs

DOI: 10.17221/146/2020-CJFS

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