Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food

Marko Dmitric, Dejan Vidanovic, Kazimir Matovic, Bojana Tesovic, Milanko Sekler, Ivan Vicic and Nedjeljko Karabasil
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Dejan Vidanovic: Veterinary Specialised Institute Kraljevo, Kraljevo, Serbia
Kazimir Matovic: Veterinary Specialised Institute Kraljevo, Kraljevo, Serbia
Bojana Tesovic: Veterinary Specialised Institute Kraljevo, Kraljevo, Serbia
Milanko Sekler: Veterinary Specialised Institute Kraljevo, Kraljevo, Serbia
Ivan Vicic: Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
Nedjeljko Karabasil: Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

Czech Journal of Food Sciences, 2023, vol. 41, issue 4, 287-294

Abstract: In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food.

Keywords: molecular detection; food safety; validation; pathogen; artificial contamination (search for similar items in EconPapers)
Date: 2023
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:41:y:2023:i:4:id:114-2022-cjfs

DOI: 10.17221/114/2022-CJFS

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