Effect of the disinfectant benzalkonium chloride on Listeria monocytogenes biofilm

Chao Yang, Xiaochen Zhang, Tao Yu, Lin Wang, Zichuan Zhu and Xinjie Fan
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Chao Yang: Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China
Xiaochen Zhang: Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China
Tao Yu: School of Biological Engineering, Xinxiang University, Xinxiang, China
Lin Wang: Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China
Zichuan Zhu: Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China
Xinjie Fan: Faculty of Tourist Management, Xinxiang Vocational and Technical College, Xinxiang, China

Czech Journal of Food Sciences, 2024, vol. 42, issue 4, 295-304

Abstract: Listeria monocytogenes is capable of forming biofilms on the food contact surfaces, increasing the risk of food contamination by this pathogen. The disinfectant benzalkonium chloride (BC) is commonly used to control L. monocytogenes in the food industry. This study aimed to investigate effects of BC on L. monocytogenes biofilms. Biofilm biomass was measured by the microplate method with crystal violet staining. Results from the broth microdilution method showed that the minimum inhibitory concentration (MIC) of BC against L. monocytogenes 10403S was 8 μg.mL-1. Sub-MICs of BC inhibited the biofilm formation and lethal concentrations of BC removed mature biofilms of L. monocytogenes 10403S. The presence of BC reduced extracellular proteins and exopolysaccharides in biofilms. Additionally, upregulation of quorum sensing gene luxS and agrBDCA and downregulation of flagellum motility genes flaA, motA, and motB were observed in the presence of BC. The BC disinfectant has an excellent anti-biofilm activity against L. monocytogenes.

Keywords: foodborne pathogen; disinfection; anti-biofilm; extracellular polymeric substances (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjf:v:42:y:2024:i:4:id:44-2024-cjfs

DOI: 10.17221/44/2024-CJFS

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