Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs

Ulrich Kurt Posselt, Philippe Barre, Gintaras Brazauskas and Lesley Beryl Turner
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Ulrich Kurt Posselt: State Plant Breeding Institute, University of Hohenheim, Stuttgart, Germany
Philippe Barre: National Institute of Agronomic Research (INRA), Forage Genetics and Breeding Unit (UGAPF), Lusignan, France
Gintaras Brazauskas: Lithuanian Institute of Agriculture, Akademija, Kédainiai, Lithuania
Lesley Beryl Turner: Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, United Kingdom

Czech Journal of Genetics and Plant Breeding, 2006, vol. 42, issue 3, 87-94

Abstract: Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).

Keywords: marker comparison; AFLP; ISSR; RAPD; SSR; Lolium perenne L (search for similar items in EconPapers)
Date: 2006
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:42:y:2006:i:3:id:3647-cjgpb

DOI: 10.17221/3647-CJGPB

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