Estimation of Fatty Acid Content in Intact Seeds of Oilseed Rape (Brassica napus L.) Lines Using Near-Infrared Spectroscopy

Radoslav Koprna, Pavel Nerušil, Oldřich Kolovrat, Vratislav Kučera and Alois Kohoutek
Additional contact information
Radoslav Koprna: OSEVA PRO Ltd., dep. Research Institute of Oilseed Crops at Opava, Opava, Czech Republic
Pavel Nerušil: Research Station of Grass Ecosystems at Jevíčko, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
Oldřich Kolovrat: OSEVA PRO Ltd., dep. Research Institute of Oilseed Crops at Opava, Opava, Czech Republic
Vratislav Kučera: Research Institute of Crop Production, Prague-Ruzyně, Czech Republic
Alois Kohoutek: Research Station of Grass Ecosystems at Jevíčko, Research Institute of Crop Production, Prague-Ruzyně, Czech Republic

Czech Journal of Genetics and Plant Breeding, 2006, vol. 42, issue 4, 132-136

Abstract: Based on NIRS (near infrared reflectance spectroscopy) measurements carried out in a collection of 262 samples of winter oilseed rape with a different content of fatty acids (FA) in oil, calibration equations for the laboratory instrument Foss-NIRSystem 6500 were developed. Calibration was focused on the possibility of screening seed samples of different composition of oleic acid (C18:1), linoleic acid (C18:2) and linolenic acid (C18:3) using NIRS analysis. The reference method was gas chromatography (GC). The content of FA in segregating F2 generations after crossing between lines with different contents of FA and lines with standard content of FA in oil ranged from 32.3 to 82.0% for C18:1, 10.2-26.8% for C18:2 and 3.3-11.8% for C18:3. The verification of a validation equation in 50 randomly selected samples of F2 generation in the year 2006 proved high correlation coefficients (r) between NIRS analysis and GC values, r = 0.86 for C18:1, r = 0.82 for C18:2 and r = 0.85 for C18:3. Non-destructive NIRS analysis enables rapid and reliable selection of materials with different composition of FA in the seed of oilseed rape (lines with desirable high content of C18:1 and low content of C18:3).

Keywords: winter oilseed rape; fatty acids; oleic acid; linoleic acid; linolenic acid; NIRS calibration (search for similar items in EconPapers)
Date: 2006
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjgpb.agriculturejournals.cz/doi/10.17221/3643-CJGPB.html (text/html)
http://cjgpb.agriculturejournals.cz/doi/10.17221/3643-CJGPB.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:42:y:2006:i:4:id:3643-cjgpb

DOI: 10.17221/3643-CJGPB

Access Statistics for this article

Czech Journal of Genetics and Plant Breeding is currently edited by Ing. Markéta Knížková, (Executive Editor)

More articles in Czech Journal of Genetics and Plant Breeding from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().