Utilization of a molecular marker for evaluation of hybridization success in spring barley genetics and breeding

Marie Špunarová, Milan Pouch and Jaroslav Špunar
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Marie Špunarová: Agrotest fyto, Ltd., Kroměříž, Czech Republic
Milan Pouch: Agrotest fyto, Ltd., Kroměříž, Czech Republic
Jaroslav Špunar: Agrotest fyto, Ltd., Kroměříž, Czech Republic

Czech Journal of Genetics and Plant Breeding, 2007, vol. 43, issue 4, 144-148

Abstract: The success in hybridization of two-rowed spring barley genotypes of various origins was verified using the codominant SSR (Simple Sequence Repeat) marker HVWAXY which is located in the coding sequence of the waxy gene. The primer pair F 5' AAG ACG TGG TGT TCG TGT G 3' and R 5' ATG GTT CCA GGG GTA AGT TC 3' generated the PCR product of approximately 200 bp in the varieties/breeding lines Maridol, Bojos, Malz, Xanadu, Isotta, Josefin, Native, Sebastian, Conrad, KM 2436, KM 2439, KM 2629, KM-H-1320, whereas the product of about 250 bp was amplified in the lines KM 2416 and Br 7571h33. The parental genotypes possessing different alleles of the marker were crossed and the segregating lines of the F3 generation were characterized employing molecular methods (marker-assisted selection). This prescreening enabled to work with a lower number of individuals (selfed individuals were excluded) at the initial stages of the breeding process leading to the acceleration of selection as a particular phase of breeding.

Keywords: barley; Hordeum vulgare L.; hybridization; marker-assisted selection; SSR marker (search for similar items in EconPapers)
Date: 2007
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:43:y:2007:i:4:id:1900-cjgpb

DOI: 10.17221/1900-CJGPB

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