Seed protein electrophoresis for assessment of genetic variation within genotypes of meadow fescue (Festuca pratensis Huds)

S. Stoyanova and B. Boller
Additional contact information
S. Stoyanova: Institute of Plant Genetic Resources, 4122 Sadovo, Bulgaria
B. Boller: Agroscope Reckenholz-Tänikon Research Station ART, 8046 Zurich, Switzerland, e-mail: s_stoyanova@gbg.bg

Czech Journal of Genetics and Plant Breeding, 2010, vol. 46, issue SpecialIssue, S75-S81

Abstract: The protein diversity of 45 genotypes (forty genotypes selected within two gene pools and five varieties) of meadow fescue was examined. Genetic variation was described using ISTA/UPOV methods for crop variety identification. Modifications of acid-PAGE of alcohol-soluble proteins (prolamins), and SDS-PAGE of salt-soluble proteins (globulins) were elaborated for seed analyses of Festuca pratensis Huds. The results of this study indicated that the genotypes of meadow fescue could effectively be differentiated on the basis of polymorphism, detected between protein patterns. SDS-PAGE presented a higher differentiation power and better repeatability; thus could be used as a rapid and reliable method for the identification of F. pratensis genotypes in breeding programmes and the seed industry.

Keywords: acid-PAGE; genetic diversity; meadow fescue; SDS-PAGE (search for similar items in EconPapers)
Date: 2010
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjgpb.agriculturejournals.cz/doi/10.17221/2569-CJGPB.html (text/html)
http://cjgpb.agriculturejournals.cz/doi/10.17221/2569-CJGPB.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:46:y:2010:i:specialissue:id:2569-cjgpb

DOI: 10.17221/2569-CJGPB

Access Statistics for this article

Czech Journal of Genetics and Plant Breeding is currently edited by Ing. Markéta Knížková, (Executive Editor)

More articles in Czech Journal of Genetics and Plant Breeding from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().