Detection of various U and M chromosomes in wheat-Aegilops biuncialis hybrids and derivatives using fluorescence in situ hybridisation and molecular markers

Annamária Schneider and Márta Molnár-Láng
Additional contact information
Annamária Schneider: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary
Márta Molnár-Láng: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary

Czech Journal of Genetics and Plant Breeding, 2012, vol. 48, issue 4, 169-177

Abstract: The aim of the study was to select wheat-Aegilops biuncialis addition lines carrying Aegilops biuncialis chromosomes differing from those which were introgressed into the wheat-Ae. biuncialis addition lines produced earlier in Martonvásár, Hungary. In the course of the experiments new wheat-Ae. biuncialis addition lines carrying chromosomes 2Ub, 6Mb, 6Ub; 5Ub, 3Ub, 7Ub; 5Mb, 6Mb and 7Mb were selected. The 2Ub disomic addition line is relatively stable, as 91% of the progenies contain this chromosome pair. The 6Mb disomic addition line proved to be dwarf and sterile, but it still exists as a monosomic addition line. Progenies analysed from the 6Ub monosomic addition line did not carry the 6Ub chromosome. One plant containing the 5Ub, 3Ub and 7Ub chromosomes and one plant carrying 5Mb, 6Mb and 7Mb chromosomes showed very low fertility. Each of the plants produced a single seed, but seeds of the parent plants are still available. Line No. 49/00 carried a submetacentric Ae. biuncialis chromosome pair and the chromosome number 44 has been constant for several generations. After FISH no hybridisation site was observed on the Ae. biuncialis chromosome pair using the pSc119.2 and Afa family repetitive DNA probes, so it was not possible to identify the Ae. biuncialis chromosome pair. However, the use of wheat SSR markers and the (GAA)n microsatellite DNA probe allowed it to be characterised more accurately. These new lines facilitate gene transfer from Ae. biuncialis into cultivated wheat and the selection of U and M genome-specific wheat SSR markers.

Keywords: addition lines; FISH polymorphism; goatgrass; wheat SSR markers (search for similar items in EconPapers)
Date: 2012
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjgpb.agriculturejournals.cz/doi/10.17221/45/2012-CJGPB.html (text/html)
http://cjgpb.agriculturejournals.cz/doi/10.17221/45/2012-CJGPB.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:48:y:2012:i:4:id:45-2012-cjgpb

DOI: 10.17221/45/2012-CJGPB

Access Statistics for this article

Czech Journal of Genetics and Plant Breeding is currently edited by Ing. Markéta Knížková, (Executive Editor)

More articles in Czech Journal of Genetics and Plant Breeding from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().