Detection of QTLs for important agronomical traits in hexaploid wheat using association analysis

Ljiljana Brbaklic, Dragana Trkulja, Ankica Kondic-Spika, Sanja Treskic and Borislav Kobiljski
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Ljiljana Brbaklic: Small Grains Department, Institute of Field and Vegetable Crops, Novi Sad, Serbia
Dragana Trkulja: Small Grains Department, Institute of Field and Vegetable Crops, Novi Sad, Serbia
Ankica Kondic-Spika: Small Grains Department, Institute of Field and Vegetable Crops, Novi Sad, Serbia
Sanja Treskic: Small Grains Department, Institute of Field and Vegetable Crops, Novi Sad, Serbia
Borislav Kobiljski: Small Grains Department, Institute of Field and Vegetable Crops, Novi Sad, Serbia

Czech Journal of Genetics and Plant Breeding, 2013, vol. 49, issue 1, 1-8

Abstract: One of the main wheat breeder's goals is determining specific genomic regions which control important agronomical traits. Association analysis is a new strategy with high resolution in plant molecular breeding that could be used to improve the efficiency of marker assisted selection (MAS) for finding important QTLs (quantitative trait loci) or genes. A set of 96 diverse wheat genotypes was phenotypically measured during three growing seasons (2006/07, 2007/08, 2008/09). Microsatellite markers located near important QTLs were carefully chosen in accordance with existing literature data to validate marker trait associations (MTA). Genomic DNA was extracted using the CTAB method and PCR products were separated by capillary electrophoresis. The population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by the Tassel program using the Q matrix. Nine significant associations were stable in all years investigated and eight MTA were detected to be significant in two growing seasons. Microsatellite markers which showed significant associations and stability in different seasons can be useful and suitable for marker assisted selection (MAS) in Serbian wheat breeding programs.

Keywords: agronomical traits; microsatellite markers; QTLs; Tassel; Triticum aestivum (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:49:y:2013:i:1:id:64-2012-cjgpb

DOI: 10.17221/64/2012-CJGPB

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