Integration and characterization of T-DNA insertion in upland cotton

Xiaojie Yang, Fuguang Li, Xueyan Zhang, Kun Liu, Qianhua Wang, Chaojun Zhang, Chuanliang Liu, Wei Zhu, Guofang Shan, Chee-Kok Chin and Weiping Fang
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Xiaojie Yang: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Fuguang Li: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Xueyan Zhang: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Kun Liu: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Qianhua Wang: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Chaojun Zhang: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Chuanliang Liu: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Wei Zhu: Agronomy College of Henan Agricultural University, Zhengzhou, P.R. China
Guofang Shan: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agriculture Sciences (CAAS), Anyang, Henan, P.R. China
Chee-Kok Chin: Department of Plant Science, Rutgers University, New Jersey, USA
Weiping Fang: Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, P.R. China

Czech Journal of Genetics and Plant Breeding, 2013, vol. 49, issue 2, 51-57

Abstract: Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed co-transformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the inner region of RB. The distribution of T-DNA insertions in upland cotton genome included coding sequences, transposons, plastid-derived sequences and microsatellites.

Keywords: cotton (Gossypium hirsutum L.); deletion of border; genetic transformation; transgene copy; vector integration (search for similar items in EconPapers)
Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:49:y:2013:i:2:id:217-2012-cjgpb

DOI: 10.17221/217/2012-CJGPB

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