Identification of an active 1Ay gene from Triticum turgidum ssp. dicoccoides

Zhe-Guang Bi, Bi-Hua Wu, Xi-Gui Hu, Xiao-Hui Guo, Deng-Cai Liu and You-Liang Zheng
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Zhe-Guang Bi: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
Bi-Hua Wu: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
Xi-Gui Hu: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
Xiao-Hui Guo: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
Deng-Cai Liu: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China
You-Liang Zheng: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, P.R. China

Czech Journal of Genetics and Plant Breeding, 2014, vol. 50, issue 3, 208-215

Abstract: The high molecular weight glutenin subunit (HMW-GS), encoded by the 1Ay gene, unexpressed in common wheat, exists in diploid and tetraploid wheats. An active 1Ay gene was first cloned from wild emmer wheat (T. turgidum ssp. dicoccoides, 2n = 4x = 28, AABB), the oldest species in emmer wheat. Here, a novel subunit encoded by the 1Ay gene (JF519636) present in T. turgidum ssp. dicoccoides line D141 was characterized. The protein had 608 amino acids with six cysteine residues and showed faster electrophoretic mobility than 1Dy12 from common wheat. Compared with previously reported 1Ay subunits, it contained 16 single point mutations (SPMs). Comparative and phylogenetic sequence analyses suggested that this gene was more similar to the 1Ay gene from the diploid species (2n = 2x = 14, AA) T. urartu than from T. monococcum ssp. aegilopoides. Its predicted secondary protein structure possessed a different content of motifs relative to the 1Ay gene (AY245578) from T. urartu, which had similar electrophoretic mobility. In the central repetitive domain, JF519636 had more β-turns and β-bends than the 1Ay subunit AY245578. These structural characteristics in JF519636 could possibly be associated with specific gluten properties.

Keywords: 1Ay gene; high molecular weight glutenin subunit (HMW-GS); phylogenetic analysis; wild emmer wheat (search for similar items in EconPapers)
Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:50:y:2014:i:3:id:195-2012-cjgpb

DOI: 10.17221/195/2012-CJGPB

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