A rapid and cost-effective procedure for delineation and utilization of genomic microsatellites for paralleled genotyping in Vicia faba

Jelena M. Aleksić, Bojana Banović, Jovanka Miljuš-Đukić, Živko Jovanović, Aleksandar Mikić, Branko Ćupina, Bojan Zlatković, Snežana Anđelković, Ilaria Spanu, Mihailo Jelić and Vesna Maksimović
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Jelena M. Aleksić: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia
Bojana Banović: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia
Jovanka Miljuš-Đukić: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia
Živko Jovanović: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia
Aleksandar Mikić: Institute of Field and Vegetable Crops (IFVCNS), Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia
Branko Ćupina: Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia
Bojan Zlatković: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Niš, Serbia
Snežana Anđelković: Institute for Forage Crops (IFC), Kruševac, Serbia
Ilaria Spanu: Institute of Biosciences and Bioresources (IBBR) UOS, Sesto Fiorentino, Florence, Italy
Mihailo Jelić: Department of Genetics and Evolution, Faculty of Biology, University of Belgrade, Belgrade, Serbia
Vesna Maksimović: Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia

Czech Journal of Genetics and Plant Breeding, 2015, vol. 51, issue 1, 36-39

Abstract: Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.

Keywords: faba bean; genomic SSRs; multiplexing; Type-it kit (search for similar items in EconPapers)
Date: 2015
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:51:y:2015:i:1:id:153-2014-cjgpb

DOI: 10.17221/153/2014-CJGPB

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