Identification of genes conferring resistance to viral diseases of barley using multiplex PCR

Tibor Sedláček, Pavel Mařík and Jana Chrpová
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Tibor Sedláček: Research Centre SELTON, Ltd., Stupice, Czech Republic
Pavel Mařík: Research Centre SELTON, Ltd., Stupice, Czech Republic
Jana Chrpová: Crop Research Institute, Prague-Ruzyně, Czech Republic

Czech Journal of Genetics and Plant Breeding, 2016, vol. 52, issue 1, 30-33

Abstract: This paper describes a rapid and cost-effective assay for identifying economically important barley resistance genes based on multiplex PCR. A newly created reaction mixture is used for detecting the resistance genes rym4, rym5, rym11-b and Ryd2 conferring resistance to barley yellow mosaic viruses and Barley yellow dwarf virus (BYDV). It is possible to identify all these genes using only a single PCR reaction without the necessity for subsequent cutting with restriction endonucleases. This is important for developing resistant barley genotypes inasmuch as it can increase work productivity and reduce costs.

Keywords: BaYMV; BaMMV; BaYMV-2; BYDV; Hordeum; resistance genes (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:52:y:2016:i:1:id:171-2015-cjgpb

DOI: 10.17221/171/2015-CJGPB

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