Two-dimensional root phenotyping system based on root growth on black filter paper and recirculation micro-irrigation

Wutthida Rattanapichai and Karel Klem
Additional contact information
Wutthida Rattanapichai: Department of Agrosystems and Bioclimatology, Mendel University in Brno, Brno, Czech Republic
Karel Klem: Global Change Research Institute CAS, Brno, Czech Republic

Czech Journal of Genetics and Plant Breeding, 2016, vol. 52, issue 2, 64-70

Abstract: Plant root system architecture (RSA) has an important role in crop production, particularly for water and nutrient uptake under limiting conditions. In the last few years, several root phenotyping methods have been developed. Here we present a new technique which has been developed for non-destructive, inexpensive and high-throughput root growth studies and RSA analyses. To illustrate the potential applications, this method was tested in an experiment with nitrogen and phosphorous deficiencies in a nutrient solution, affecting RSA parameters of two spring barley varieties (Bojos and Barke). This technique is based on root growth on vertically positioned black filter paper (30 × 60 cm) placed between two black plastic (PVC-P) foils and micro-irrigation systems providing the recirculation of nutrient solution. The pre-germinated seeds were placed in the slit between two plastic bars which carry the filter paper and plastic sheets and fix the plant in the vertical position. This system allows easy repeated non-invasive access to roots for their measuring and sampling. Eighteen days after transplanting the root imaging was done using an RGB digital camera. To evaluate the root architecture parameters the "SmartRoot" software was used. The results revealed that the system is able to detect changes in RSA which are caused mainly by P deficiency (particularly changes in lateral root length and total root area). It can be concluded that this technique has a great potential for non-destructive root growth studies, RSA measurement and root sampling.

Keywords: image analysis; nutrient deficiency; root system architecture; spring barley (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjgpb.agriculturejournals.cz/doi/10.17221/121/2015-CJGPB.html (text/html)
http://cjgpb.agriculturejournals.cz/doi/10.17221/121/2015-CJGPB.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:52:y:2016:i:2:id:121-2015-cjgpb

DOI: 10.17221/121/2015-CJGPB

Access Statistics for this article

Czech Journal of Genetics and Plant Breeding is currently edited by Ing. Markéta Knížková, (Executive Editor)

More articles in Czech Journal of Genetics and Plant Breeding from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().