Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene

Gang Xue, Li-Ming Lu, Tie-Zhao Yang, Xiao-Hui Li, Xue-Xia Xing and Shi-Xiao Xu
Additional contact information
Gang Xue: College of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. China
Li-Ming Lu: College of Agronomy, Sichuan Agricultural University, Ya'an, P.R. China
Tie-Zhao Yang: College of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. China
Xiao-Hui Li: College of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. China
Xue-Xia Xing: College of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. China
Shi-Xiao Xu: College of Tobacco Science, Henan Agricultural University, Zhengzhou, P.R. China

Czech Journal of Genetics and Plant Breeding, 2016, vol. 52, issue 2, 77-82

Abstract: Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family, AtCIPK23, in low-K+ stress responses in tobacco. Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased low-K+ tolerance, which was demonstrated by higher dry biomass, longer primary root length, higher K+ content and better growth status of transgenic tobacco plants compared to controls when both were treated in low-K+ MS medium and low-K+ hydroponics. Moreover, transgenic lines conferred tolerance to low-K+ stress by increasing the K+ uptake rate under low-K+ conditions. Taken together, these results provide evidence that AtCIPK23 may be involved in the CBL-CIPK signalling network in tobacco responses to low-K+ stress.

Keywords: AtCIPK23; K+ uptake; low-K+ tolerance; potassium; tobacco (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjgpb.agriculturejournals.cz/doi/10.17221/155/2015-CJGPB.html (text/html)
http://cjgpb.agriculturejournals.cz/doi/10.17221/155/2015-CJGPB.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:52:y:2016:i:2:id:155-2015-cjgpb

DOI: 10.17221/155/2015-CJGPB

Access Statistics for this article

Czech Journal of Genetics and Plant Breeding is currently edited by Ing. Markéta Knížková, (Executive Editor)

More articles in Czech Journal of Genetics and Plant Breeding from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().