Study of informative DNA markers of the Rf1 gene in sunflower for breeding practice

Nikolay Markin, Alexander Usatov, Maksim Makarenko, Kiril Azarin, Oleg Gorbachenko, Natalia Kolokolova, Tatyana Usatenko, Olga Markina and Vera Gavrilova
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Nikolay Markin: Southern Federal University, Rostov-on-Don, Russia
Alexander Usatov: Southern Federal University, Rostov-on-Don, Russia
Maksim Makarenko: Southern Federal University, Rostov-on-Don, Russia
Kiril Azarin: Southern Federal University, Rostov-on-Don, Russia
Oleg Gorbachenko: L.A. Zhdanov Don Experimental Station of the All-Russian Institute of Oil Plants, Oporny, Rostov Region, Russia
Natalia Kolokolova: Southern Federal University, Rostov-on-Don, Russia
Tatyana Usatenko: L.A. Zhdanov Don Experimental Station of the All-Russian Institute of Oil Plants, Oporny, Rostov Region, Russia
Olga Markina: Southern Federal University, Rostov-on-Don, Russia
Vera Gavrilova: N.I. Vavilov Institute of Plant Genetic Resources, St. Petersburg, Russia

Czech Journal of Genetics and Plant Breeding, 2017, vol. 53, issue 2, 69-75

Abstract: The investigation of DNA markers associated with the pollen fertility restoration gene (Rf1) was conducted in Helianthus. Two sequence-characterized amplified region (SCAR) markers - HRG01 and HRG02 were informative for the identification of Rf1 gene in selections of sunflower plants. The codominant character of the HRG01 marker and HRG01 amplicon polymorphism has been determined. Five annual and twenty-six perennial species of sunflower were tested for the presence of Rf genotypes. HRG02 proved to be a more appropriate marker for Rf1 determination in perennial species, and HRG01 was more informative for annual species. We have also developed the multiplex RT-PCR test system, which allows simultaneously detecting the dominant allele of Rf1 and CMS-PET1 associated mitotype.

Keywords: cytoplasmic male sterility (CMS); DNA-based markers; fertility restorer genes; Helianthus; testing system (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:53:y:2017:i:2:id:108-2016-cjgpb

DOI: 10.17221/108/2016-CJGPB

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