Genetic analysis and fine mapping of the RK4 gene for round kernel in rice (Oryza sativa L.)

Shengqiang Li, Ruiyue Zhang, Jipeng Chen, Jie Zou, Tao Liu and Guohua Zhou
Additional contact information
Ruiyue Zhang: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China
Jipeng Chen: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China
Jie Zou: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China
Tao Liu: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China
Guohua Zhou: College of Life Science and Environmental Resources, Yichun University, Yichun, Jiangxi, P.R. China

Czech Journal of Genetics and Plant Breeding, 2017, vol. 53, issue 4, 153-158

Abstract: Grain shape of rice is an important trait for both yield and quality. A rice rk4 (round kernel) mutant was obtained from the japonica variety Zhonghua 11 by radiation of 60Co-γ. The grain width of the mutant was increased and the length was decreased. Simultaneously, the 1000-grain weight was slightly reduced, therefore the grain shape of the mutant tended to be small and round. In this study, genetic analysis and gene mapping of the mutant gene were carried out using the F2 and F3 populations derived from the mutant and the indica variety Xianhui 8006. The results suggested that the round kernel was controlled by a single recessive allele (rk4) which was located on chromosome 5. The RK4 gene was further mapped between the molecular markers LSTS5-77 and LSTS5-60 with 0.57 and 0.096 cM, respectively. A BAC clone was found to span the RK4 locus, and the RK4 gene was placed in a 46 kb region that contains six annotated genes according to the available sequence annotation database. This result will help us to isolate the RK4 gene and reveal the molecular mechanism of the round kernel in rice.

Keywords: BSA; ESEM; kernel shape; SSR; STS (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
http://cjgpb.agriculturejournals.cz/doi/10.17221/172/2016-CJGPB.html (text/html)
http://cjgpb.agriculturejournals.cz/doi/10.17221/172/2016-CJGPB.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:53:y:2017:i:4:id:172-2016-cjgpb

DOI: 10.17221/172/2016-CJGPB

Access Statistics for this article

Czech Journal of Genetics and Plant Breeding is currently edited by Ing. Markéta Knížková, (Executive Editor)

More articles in Czech Journal of Genetics and Plant Breeding from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().