Lignin biosynthesis regulated by the antisense 4CL gene in alfalfa

Jing Meng, Caona Li, Manlin Zhao, Cheng Wang, Yi Ru, Zhixin Cui and Yang Han
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Jing Meng: School of Life Science, Liaoning University, Shenyang, P.R. China
Caona Li: School of Life Science, Liaoning University, Shenyang, P.R. China
Manlin Zhao: School of Life Science, Liaoning University, Shenyang, P.R. China
Cheng Wang: School of Life Science, Liaoning University, Shenyang, P.R. China
Yi Ru: School of Life Science, Liaoning University, Shenyang, P.R. China
Zhixin Cui: School of Life Science, Liaoning University, Shenyang, P.R. China

Czech Journal of Genetics and Plant Breeding, 2018, vol. 54, issue 1, 26-29

Abstract: The Antisense 4CL gene was transfected into alfalfa through Agrobacterium-mediated transfer. The test results indicated that the antisense 4CL gene was successfully integrated into the genome DNA of alfalfa and was stably transmitted to the offspring. Compared to the wild-type plants, the lignin content of T0 and T1 generation plants was reduced by 45.77% and 31.97%, respectively; there were no significant differences in height and weight of T0 and T1 plants, compared to the wild-type plants. However, the transgenic plant differed from the wild-type plant by softer stems and leaves, larger leaves, fewer flowers and a fewer seeds. The T0 line was susceptible to disease infection, but significantly improved in the second year. The results suggest that the 4CL gene from Amorpha fruticosa can be used to regulate lignin biosynthesis in transgenic forage crops.

Keywords: antisense recombinant plasmid; 4-coumarate coenzyme A ligase; lignin content; Medicago sativa (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:54:y:2018:i:1:id:23-2017-cjgpb

DOI: 10.17221/23/2017-CJGPB

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