In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation

Pranit Kumar Mukherjee, Raju Mondal, Sourav Dutta, Kanti Meena, Madhumita Roy and Asit Baran Mandal
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Pranit Kumar Mukherjee: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India
Raju Mondal: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India
Sourav Dutta: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India
Kanti Meena: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India
Madhumita Roy: Department of Biotechnology, Techno India University, Kolkata, West Bengal, India
Asit Baran Mandal: ICAR - Central Research Institute for Jute & Allied Fibres, Barrackpore, West Bengal, India

Czech Journal of Genetics and Plant Breeding, 2018, vol. 54, issue 4, 183-189

Abstract: An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO3. 1/2 Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological character in comparison with the parental stock. Furthermore, clonal fidelity of the mericlones was confirmed by using DNA markers (random amplified polymorphic DNA and inter simple sequence repeats) and by polypeptide profiling through SDS-PAGE at a genomic and protein level, respectively, which showed the true-to-type nature of the in vitro micropropagated plants. Thus the protocol developed can be used to generate safe planting material for large-scale cultivation of ramie.

Keywords: clonal fidelity; molecular markers; ramie; tissue culture; vegetative propagation (search for similar items in EconPapers)
Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:54:y:2018:i:4:id:79-2017-cjgpb

DOI: 10.17221/79/2017-CJGPB

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