Efficiency of three haplomethods in durum wheat (Triticum turgidum subsp. durum Desf.): isolated microspore culture, gynogenesis and wheat × maize crosses

Olfa Slama-Ayed, Imen Bouhaouel, Sourour Ayed, Jacques De Buyser, Emmanuel Picard and Hajer Slim Amara
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Olfa Slama-Ayed: Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia
Imen Bouhaouel: Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia
Sourour Ayed: Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia
Jacques De Buyser: MVEH Laboratory, Paris-Sud University, Orsay, France
Emmanuel Picard: MVEH Laboratory, Paris-Sud University, Orsay, France
Hajer Slim Amara: Genetic and Cereal Breeding Laboratory, National Agronomic Institute of Tunisia, University of Carthage, Tunis Mahrajene, Tunisia

Czech Journal of Genetics and Plant Breeding, 2019, vol. 55, issue 3, 101-109

Abstract: This study presents the first report comparing the efficiency of microspore culture, gynogenesis and durum wheat × maize crosses for haploid plant production from three durum wheat genotypes (Razzek, Karim and Jneh Khotifa). The results showed that the best induction, calli or embryos formation and plant regeneration rates for the three genotypes were obtained with gynogenesis (47.2, 7.6, 0.8%), followed by interspecific crosses (33.1, 1.7, 0.4%) and isolated microspore culture (8.2, 0.05, 0.01%). Interestingly, all plants regenerated by gynogenesis and durum wheat × maize crosses were green whereas all plants obtained by isolated microspore culture were albino. In the haploid production system, all steps of the process are important for the three methods. The critical steps that have greatly reduced the number of regenerated haploid plants were induction, embryogenesis and regeneration for microspore culture, forming and regeneration of calli or embryo and haploid regeneration for interspecific crosses and gynogenesis. Genotypes with good capacity of induction have not necessarily a good capacity of haploid plantlets regeneration and vice-versa. However, calli or embryos formation seems to be an indicator of the haploid production. Overall, Razzek showed a good ability to produce haploids using the three methods. Each haplomethod showed a specific advantage. Although gynogenesis is the less used method for durum wheat, it has proved to be a successful approach for green haploid plant production.

Keywords: androgenesis; haploidization; interspecific crosses; unpollinated ovary culture; wheat (search for similar items in EconPapers)
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:55:y:2019:i:3:id:188-2017-cjgpb

DOI: 10.17221/188/2017-CJGPB

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